Online pcr calculator tool

comprehensive primer test, the melting temperature calculation for standard and degenerate oligonucleotides including LNA and other modifications, primer PCR   unique, group-specific, and in silico (virtual) PCR analysis; temperature melting (Tm), annealing (Ta) calculation, dimer prediction calculator, analyzes different 

Perform calculations for large scale PCR reactions and ensure success of scaled up reactions with this online PCR Master Mix Calculator. OligoCalc: an online oligonucleotide properties calculator - (Reference: W.A. This Web server tool automates the design of optimal PCR primers for several  We are pleased to offer OligoEvaluator™, our online oligonucleotide sequence calculator that provides primer analysis values for PCR: Base count; Molecular  comprehensive primer test, the melting temperature calculation for standard and degenerate oligonucleotides including LNA and other modifications, primer PCR  

PCR Master Mix Calculator. Performing calculations for large scale PCR reactions can be cumbersome and tedious. Ensure your success of scaled up reactions by using the PCR Master Mix Calculator. This online tool will calculate the amounts of components needed to create your PCR Master Mix. PCR Master Mix Calculator.

The OligoAnalyzer Tool gives you critical insight into the behavior and properties of your desired sequences, before you order. ✔ Length. ✔ GC content. ✔ Melting temperature (T m ) ✔ Molecular weight. ✔ Secondary structure. Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products. NEB Tm Calculator (tmcalculator.neb.com) NEB Tm Calculator ABOUT THIS TOOL. The calculator calculates recommended T m (melting temperature) of primers and PCR annealing temperature based on the primer pair sequence, primer concentration, and DNA polymerase used in PCR. The calculator also calculates the primer length, percentage of GC content, molecular weight, and extinction coefficient. The maximum number of PCR targets (amplicons) to be shown when checking specificity for pre-designed primers. The maximum number of PCR targets (amplicons) to be found on any single sequence in the search database. The number of consecutive Gs and Cs at the 3' end of both the left and right primer.

Sigma is pleased to offer OligoEvaluator, our online oligonucleotide sequence calculator. OligoEvaluator is easy to use: register or login, select DNA or RNA, paste your sequence, and click calculate. All reported properties are available for export to a convenient Excel template.

Search for information about pangenomic oligonucleotide microarray probe sets. OligoCalc -- An online oligonucleotide properties calculator. Calculate DNA and   The PCR Suite, It's an online primer designing software, hosted by UCSC, that Online tool to calculate several features (physical constants, Thermodynamic  Lincoln Stein championed the idea of making Primer3 a software component suitable for high-throughput primer design. Web interface by Steve Rozen 

OligoCalc: an online oligonucleotide properties calculator - (Reference: W.A. This Web server tool automates the design of optimal PCR primers for several 

PCR Master Mix Calculator. Performing calculations for large scale PCR reactions can be cumbersome and tedious. Ensure your success of scaled up reactions by using the PCR Master Mix Calculator. This online tool will calculate the amounts of components needed to create your PCR Master Mix. PCR Master Mix Calculator. Tm Calculator Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions. This calculator gives the amplification efficiency of a qPCR reaction based on the slope of the standard curve. Enter the slope of your standard curve into one of the two calculators below (depending on whether Ct is on the x- or y-axis of your graph). Amplification factor = 2.00 Amplification factor = 2.00 For BiSearch Primer Design and Search Tool - this is a useful tool for primer-design for any DNA template and especially for bisulfite-treated genomes. The ePCR tool provides fast detection of mispriming sites and alternative PCR products in cDNA libraries and native or bisulfite-treated genomes. This tool allows to calculate the content of a Trusted Platform Module (TPM) Platform Configuration Register (PCR) the way a TPM would do it. This tool also allows to perform different kinds of hash calculations.

Sigma is pleased to offer OligoEvaluator, our online oligonucleotide sequence calculator. OligoEvaluator is easy to use: register or login, select DNA or RNA, paste your sequence, and click calculate. All reported properties are available for export to a convenient Excel template.

PCR Products The program searches for perfectly matching primer annealing sites that can generate a PCR product. Any resulting products are sorted by size, and they are given a title specifying their length, their position in the original sequence, and the primers that produced them. The OligoAnalyzer Tool gives you critical insight into the behavior and properties of your desired sequences, before you order. ✔ Length. ✔ GC content. ✔ Melting temperature (T m ) ✔ Molecular weight. ✔ Secondary structure. Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products. NEB Tm Calculator (tmcalculator.neb.com) NEB Tm Calculator ABOUT THIS TOOL. The calculator calculates recommended T m (melting temperature) of primers and PCR annealing temperature based on the primer pair sequence, primer concentration, and DNA polymerase used in PCR. The calculator also calculates the primer length, percentage of GC content, molecular weight, and extinction coefficient. The maximum number of PCR targets (amplicons) to be shown when checking specificity for pre-designed primers. The maximum number of PCR targets (amplicons) to be found on any single sequence in the search database. The number of consecutive Gs and Cs at the 3' end of both the left and right primer. FastPCR and jPCR provides comprehensive facilities for designing primers (oligonucleotide, oligo, probe) for most PCR applications including standard, multiplex, long distance, inverse, real-time, unique, group-specific, and in silico (virtual) PCR analysis; temperature melting (Tm), annealing (Ta) calculation, dimer prediction calculator, analyzes different features of multiple primers simultaneously, Tm, GC content, dimer formation and else; optimal Ta (temperature of annealing PCR Box Titration Calculator (Attotron Biosensor Corporation) This JavaScript program calculates the amounts of various ingredients needed to do two-dimensional box titrations of various reactants in polmerase chain reactions.

The calculator calculates recommended T m (melting temperature) of primers and PCR annealing temperature based on the primer pair sequence, primer concentration, and DNA polymerase used in PCR. The calculator also calculates the primer length, percentage of GC content, molecular weight, and extinction coefficient.